About Us
News
Announcement
Research
Conservation & Horticulture
Public Education
Graduate Study
Scientist
International Cooperation
Resources
Annual Reports
Publications & Papers
Visit XTBG
Societies
XTBG Seminar
Open Positions
4th XSBN Symposium
CAS-SEABRI
PFS-Tropical Asia
Links
 
   Location:Home > Research > Research Progress
19 microsatellite markers developed for studying genetics and mating system of D. pavettifolia
Author: Lin Hua
ArticleSource:
Update time: 2012-10-31
Close
Text Size: A A A
Print

Duperrea pavettifolia is only one of the species D. pavettifolia (Kurz) Pit, a small genus of the Rubiaceae with only two species, found in China. It has prominent long exserted styles and swollen stigmas with abundant pollen. It is a self-compatible species. To understand its mating system analysis and conservation research, the development of microsatellite primers will be fundamental.

Dr. Lin Hua and her colleagues of Xishuangbanna Tropical Botanical Garden (XTBG) developed a range of microsatellites to investigate whether D. pavettifolia are predominantly self-pollinating or outcrossers. They used microsatellite markers to characterize D. pavettifolia to further study genetic diversity, population structure, mating system, and conservation.

 In total, 19 microsatellite markers were developed and characterized in two wild populations by using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol.

The microsatellite markers developed in their study were suitable for the fine-scale understanding of population genetic structure. They will be useful to further study the mating systems of D. pavettifolia, a secondary pollen presenting plant, and to establish a conservation strategy.

The study entitled “Microsatellite markers for Duperrea pavettifolia (Rubiaceae)” has been published in American Journal of Botany, 99(8):e310-312, doi: 10.3732/ajb.1100609

  Appendix Download
Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences. Menglun, Mengla, Yunnan 666303, China
Copyright XTBG 2005-2014 Powered by XTBG Information Center