| Title | Desiccation tolerance and cryopreservation of Archontophoenix alexandrae excised embryos at different developmental stages |
| Author | Shao, Y. T. |
| Publication | SEED SCIENCE AND TECHNOLOGY |
| Year | 2009 |
| Volume | 37 |
| Issue | 1 |
| Corresponding Author | Yin, SH |
| Abstract | This paper describes research results on relative electrolyte leakage. survival, seedling, rate, desiccation tolerance and cryopreservation of Archontophoenix alexandrae excised embryos during development. Desiccation tolerance of excised embryos increased gradually from 146 DAF (days after flowering) to 174 DAF and reached the maximum at 174 DAF with a semilethal water content of 0.08 but decreased at 181 DAF Excised embryos of 146-153 DAF with/without water content treatments failed to survive after cryopreservation. but excised embryos of 160-181 DAF drying, to suitable water Content survived after desiccation and freezing. Drying to 0.25 g g(-1) (dry weight), survival of excised embryos of 160, 167, 174, 181 DAF was 56%. 63%, 98% and 46% respectively after cryopreservation. Excised embryos formed seedling, but the plantlet formation percentage was lower than the growth rate of seedling. Roots meristems appeared to be more sensitive to desiccation and freezing than shoot meristems. Seeds with/without water content treatments failed to survival after cryopreservation. |
