| Title | Characterization, Expression Profiling and Heterologous Function Analysis of Two Oleosin Genes PvOle1 and PvOle2 from Sacha Inchi (Plukenetia volubilis) |
| Author | Chandrasekaran, Umashankar |
| Publication | INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY |
| Year | 2013 |
| Volume | 15 |
| Issue | 3 |
| Corresponding Author | Liu, AZ |
| Abstract | Sacha Inchi (Plukenetia volubilis L., Euphothiaceae) is a potential oilseed crop for its wealth of seed oil rich in omega-3 fatty acids. The present study describes the isolation and characterization of two Sacha Inchi oleosin genes (PvOle1 and PvOle2) encoding oleosin proteins. Full length cDNAs of PvOle1 and PvOle2 were comprised of 829 and 763 nucleotides, encoding 143 and 139 amino acids with a molecular mass of 15.2 kDa and 14.8 kDa, respectively. Comparative and bioinformatic analysis revealed the divergence of PvOle1 and PvOle2 in their amino acid sequence and hydrophobic profiles and also the L-form of both oleosin proteins. Phylogenetic analysis indicated the possible occurrence of duplication (PvOle1 and PvOle2) before the speciation of Sacha Inchi. Semi-quantitative RT-PCR expression analysis revealed the high expression of PvOle1 and PvOle2 transcripts in the developing seeds. Further, using yeast as a heterologous expression system, we investigated the effective role of PvOle1 and PvOle2 in yeast lipid body accumulation, which was confirmed by SDS-PAGE and Immunoblotting analysis. Taken together, the characterization, expression profiling and heterologous functional analysis of two oleo sin genes (PvOle1 and PvOle2) will be helpful for further studies concerning the role of oleosin proteins in metabolic protein engineering in Sacha Inchi. (C) 2013 Friends Science Publishers |
