| Title | Determination of oil contents in Sacha inchi (Plukenetia volubilis) seeds at different developmental stages by two methods: Soxhlet extraction and time-domain nuclear magnetic resonance |
| Author | Niu, Longjian |
| Publication | INDUSTRIAL CROPS AND PRODUCTS |
| Year | 2014 |
| Volume | 56 |
| Corresponding Author | Xu, ZF |
| Abstract | Sacha inchi (Plukenetia volubilis) seeds are rich in oil that contains a high content of polyunsaturated fatty acids. For genetic improvement and breeding of Sacha inchi, a rapid and nondestructive method for determination of oil content in seeds is critical. In this study, time-domain nuclear magnetic resonance (TD-NMR) was employed to determine oil content in Sacha inchi seeds at different developmental stages, in comparison with the standard Soxhlet extraction method. A highly significant correlation (R = 0.988) between oil content determined by TD-NMR analysis and that measured by Soxhlet extraction was found. Compared with Soxhlet extraction, the TD-NMR analysis provides a more accurate determination of oil content in Sacha inchi seeds at the late period of oil accumulation. The results demonstrated that the TD-NMR analysis is a non-destructive and efficient method for reliable determination of oil content in Sacha inchi seeds at different developmental stages. (C) 2014 Elsevier B.V. All rights reserved. |
